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Equipment: Fluorescence spectrophotometer and Fluorescence software.
Apparatus: Vol. flasks 100ml;
micro burettes 10ml; burettes 50ml; pipette 5ml grad.
Sample: Purchase bottle of
commercial Tonic Water. Dilution is 1 : 50
Chemicals: Quinine sulphate
monohydrate, 90%, FW = 764.95
Sulphuric acid H2SO4
1. Quinine sulphate std: 10mg / liter. Dissolve 0.01g in 1 liter
2. 0.2N H2SO4 = 0.1M = 5.6 mls in 1liter
1. Dilute Tonic Water: 1 ml to 50ml volume.
Add 0.5 ml of diluted tonic water to 25 ml of 0.2N H2SO4 in a 50 ml vol. flask and make up to the mark with deionized water.
Operation of Instrument:
Follow User Guide: obtain help and advice from Demonstrator or