Ammonia
25% w/v in water.For neutralizing ninhydrin fixer for chromatograms and numerous other applications.
Aniline-Diphenylamine
1.8% w/v aniline and 1.8% w/v diphenylamine in acidified acetone.
For detecting reducing sugars.
Antifoam
25% antifoam reagent in methylene chloride.
For preventing/dispersing foam.
Antimony Trichloride
22% w/v antimony trichloride in chloroform.
For detecting vitamin A, steroid glycosides and related compounds.
Bromocresol Green
0.04% w/v bromocresol green in ethanol.
For detecting organic acids and bases.
Chloramine-T
5% of reagent in 0.5% sodium hydroxide.
For detection of phenolic compounds.
Cupric Acetate
3% w/v cupric acetate and 15% w/v phosphoric acid.
For detecting prostaglandins.
a-Cyclodextrin
1% w/v of the reagent in ethanol.
Distinguishes saturated from unsaturated lipids.
2 Dichlorofluorescein
0.2% w/v of the reagent in ethanol.
For detecting saturated and unsaturated lipids.
4-Dimethylaminobenzaldehyde (Ehrich reagent)
1% w/v of the reagent in acidified metganol.
For detecting amines, indoles, and ergot alkaloids.
4- Dimethylaminocinnamaldehyde
0.2% w/v of the reagent in acidified ethanol.
For detecting indoles.
N,N-Dimethyl-p-phenylenediamine dihydrochloride
1% w/v of the reagent in acidified methanol.
For detecting organic peroxides.
Diphenylcarbozone/Mercuric sulphate
DPC is 0.01% in chloroform. Mercuric sulphate is 0.01% in dilute sulphuric acid.
For detection of barbiturates.
Diphenylhexatriene
0.03% of the reagent in chloroform. For detecting lipids.
Dragendorffs reagent.
0.11% potassium iodide and 0.6millimole bismuth subnitrate in 3.5Macetic acid.
For detecting alkaloids and quaternary nitrogen compounds.
Ferric chloride-potassium ferricyanide
3% solutions in 2M hydrochloric acid
For detecting aromatic amines and phenolic compounds.
Ferric chloride
2.7% w/v of salt in 2M hydrochloric acid.
For detecting phenols and phenolic acids.
Fluorescamine
0.005% of the reagent in acetone.
For detecting primary amino acids and amines.
HPT Reagent
0.025% w/v 8-hydroxy-1,3,6-pyrenetrisulphonic acid trisodium salt in methanol.
For detecting bile acids.
Iodoplatinate
0.15% w/v potassium chloroplatinate and 3% potassium iodide in dilute hydrochloric acid.
For detecting alkaloids, amines, and organic nitrogen compounds.
Iodoplatinic acid
3ml. of 10% chloroplatinic acid diluted to 100ml.with water, then add 100ml. of 6% potassium iodide.
For detection of alkaloids, amines, analgesics and biotin.
Isatin
0.2% isatin in methanol.
For detecting proline, hydroxyproline and other amino acids and peptides.
Molybdenum blue
1.3% w/v molybdenun oxide in 4.2M sulphuric acid.
For detecting phospholipids and related compounds.
Ninhydrin
0.2% w/v ninhydrin in ethanol.or in 6% acetone.
For detecting amino acids, amines, and amino sugars.(amphetamines)
Ninhydrin Fixer reagent
Acidified ethanol containing 1% saturated cupric nitrate.
Fixative for ninhydrin chromatograms.
N-4-Nitrobenzyl-N-n-propylamine hydrochloride (NBPA)
0.2 millimole reagent
For detection of isocyanates.
Orcinol Ferric Chloride (Bial's reagent)
0.9% w/v ferric chloride and 0.55% w/v orcinol in acidified ethanol.
For detecting sugars,glycosides, and sulfolipids.
Oxime (8-hydroxyquinoline)
0.25g in 8:20 etanol/water
For detection of cations, AL, Cr, Fe, Co, Ni, Mn, Zn.
Phosphormolybdic acid
5% of reagent in Propan 2-ol
For detection of Fatty acid methyl esters, keto acids, lipids, steroids, and sulphated bile acids.
Phosphoric acid / cupric acetate
3% cupric acetate w/v in 8% phosphoric acid v/v.
For detection of Phospholipids.
Rhodamine B
0.25% of regent in ethanol.
For detecting lipids, and
For detecting metals (Au, Bi, Cd, Fe, Hg, Mo, TI, V, and W.)
Rhodamine 6G
0.01% w/v rhodamine 6G in methylene chloride.
For detecting lipids.
Rubeanic acid (sodium dithioxide)
0.1% in ethanol
For detection of cations gb111A and 111B
Sulphuric acid spray reagent
5% w/v of the acid in ethanol.
Charring reagent for organic compounds. For detection of bile acids
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